Venus trap in the mouse embryo reveals distinct molecular dynamics underlying specification of first embryonic lineages.

ABSTRACT: Mammalian development begins with the segregation of embryonic and extra-embryonic lineages in the blastocyst. Recent studies revealed cell-to-cell gene expression heterogeneity and dynamic cell rearrangements during mouse blastocyst formation. Thus, mechanistic understanding of lineage specification requires quantitative description of gene expression dynamics at a single-cell resolution in living embryos. However, only a few fluorescent gene expression reporter mice are available and quantitative live image analysis is limited so far. Here, we carried out a fluorescence gene-trap screen and established reporter mice expressing Venus specifically in the first lineages. Lineage tracking, quantitative gene expression and cell position analyses allowed us to build a comprehensive lineage map

SUBMITTER: Dietrich JE 

PROVIDER: S-EPMC4552493 | biostudies-literature | 2015 Aug

REPOSITORIES: biostudies-literature