Differential activation of airway eosinophils induces IL-13-mediated allergic Th2 pulmonary responses in mice.

ABSTRACT:

Background

Eosinophils are hallmark cells of allergic Th2 respiratory inflammation. However, the relative importance of eosinophil activation and the induction of effector functions such as the expression of IL-13 to allergic Th2 pulmonary disease remain to be defined.

Methods

Wild-type or cytokine-deficient (IL-13(-/-) or IL-4(-/-) ) eosinophils treated with cytokines (GM-CSF, IL-4, IL-33) were adoptively transferred into eosinophil-deficient recipient mice subjected to allergen provocation using established models of respiratory inflammation. Allergen-induced pulmonary changes were assessed.

Results

In contrast to the transfer of untreated blood eosinophils to the lungs of recipient eosinophil deficient mice, which induced no immune/inflammatory changes either in th

SUBMITTER: Jacobsen EA 

PROVIDER: S-EPMC4552595 | biostudies-literature | 2015 Sep

REPOSITORIES: biostudies-literature