Microtubule-Mediated Inositol Lipid Signaling Plays Critical Roles in Regulation of Blebbing.

ABSTRACT: Cells migrate by extending pseudopods such as lamellipodia and blebs. Although the signals leading to lamellipodia extension have been extensively investigated, those for bleb extension remain unclear. Here, we investigated signals for blebbing in Dictyostelium cells using a newly developed assay to induce blebbing. When cells were cut into two pieces with a microneedle, the anucleate fragments vigorously extended blebs. This assay enabled us to induce blebbing reproducibly, and analyses of knockout mutants and specific inhibitors identified candidate molecules that regulate blebbing. Blebs were also induced in anucleate fragments of leukocytes, indicating that this assay is generally applicable to animal cells. After cutting, microtubules in the anucleate fragments promptly depolymerized,

SUBMITTER: Sugiyama T 

PROVIDER: S-EPMC4552846 | biostudies-literature | 2015

REPOSITORIES: biostudies-literature