Overexpression of NIMA-related kinase 2 is associated with progression and poor prognosis of prostate cancer.

ABSTRACT:

Background

The NIMA-related kinase 2 (NEK2) is a serine/threonine kinase that is involved in regulation of centrosome duplication and spindle assembly during mitosis. Dysregulation of these processes causes chromosome instability and aneuploidy, which are hallmark changes of many solid tumors. However, whether aberrant expression of NEK2 is associated with outcome of prostate cancer (PCa) patients remains to be determined.

Methods

Expression of NEK2 in human PCa cells and primary PCa tissues was assessed by quantitative RT-PCR. Expression of NEK2 in human PCa cells was depleted with siRNA. Effects of the depletion on cell proliferation, survival, and tumorigenicity were assessed both in vitro with cell cultures and in vivo with subcutaneous implantation of xenografts. In sil

SUBMITTER: Zeng YR 

PROVIDER: S-EPMC4553013 | biostudies-literature | 2015 Aug

REPOSITORIES: biostudies-literature