Functional Immunomics of the Squash Bug, Anasa tristis (De Geer) (Heteroptera: Coreidae).

ABSTRACT: The Squash bug, Anasa tristis (De Geer), is a major piercing/sucking pest of cucurbits, causing extensive damage to plants and fruits, and transmitting phytopathogens. No genomic resources to facilitate field and laboratory studies of this pest were available; therefore the first de novo exome for this destructive pest was assembled. RNA was extracted from insects challenged with bacterial and fungal immunoelicitors, insects fed on different cucurbit species, and insects from all life stages from egg to adult. All treatments and replicates were separately barcoded for subsequent analyses, then pooled for sequencing in a single lane using the Illumina HiSeq2000 platform. Over 211 million 100-base tags generated in this manner were trimmed, filtered, and cleaned, then assembled into a de nov

SUBMITTER: Shelby KS 

PROVIDER: S-EPMC4553512 | biostudies-literature | 2013 Nov

REPOSITORIES: biostudies-literature