Systems biology definition of the core proteome of metabolism and expression is consistent with high-throughput data.

ABSTRACT: Finding the minimal set of gene functions needed to sustain life is of both fundamental and practical importance. Minimal gene lists have been proposed by using comparative genomics-based core proteome definitions. A definition of a core proteome that is supported by empirical data, is understood at the systems-level, and provides a basis for computing essential cell functions is lacking. Here, we use a systems biology-based genome-scale model of metabolism and expression to define a functional core proteome consisting of 356 gene products, accounting for 44% of the Escherichia coli proteome by mass based on proteomics data. This systems biology core proteome includes 212 genes not found in previous comparative genomics-based core proteome definitions, accounts for 65% of known essential g

SUBMITTER: Yang L 

PROVIDER: S-EPMC4553782 | biostudies-literature | 2015 Aug

REPOSITORIES: biostudies-literature