Ornithine-δ-Aminotransferase Inhibits Neurogenesis During Xenopus Embryonic Development.

ABSTRACT:

Purpose

In humans, deficiency of ornithine-δ-aminotransferase (OAT) results in progressive degeneration of the neural retina (gyrate atrophy) with blindness in the fourth decade. In this study, we used the Xenopus embryonic developmental model to study functions of the OAT gene on embryonic development.

Methods

We cloned and sequenced full-length OAT cDNA from Xenopus oocytes (X-OAT) and determined X-OAT expression in various developmental stages of Xenopus embryos and in a variety of adult tissues. The phenotype, gene expression of neural developmental markers, and enzymatic activity were detected by gain-of-function and loss-of-function manipulations.

Results

We showed that X-OAT is essential for Xenopus embryonic development, and overexpression of X-OAT produces a

SUBMITTER: Peng Y 

PROVIDER: S-EPMC4554259 | biostudies-literature | 2015 Apr

REPOSITORIES: biostudies-literature