Impact of the cryopreservation technique and vascular bed on ovarian tissue transplantation in cynomolgus monkeys.

ABSTRACT:

Purpose

The aim of this study was to determine the best combination in terms of cryopreservation techniques and vascular bed preparation before grafting in order to obtain functional ovarian tissue after transplantation.

Methods

Five cynomolgus monkeys were used. Strips from 10 ovaries were cryopreserved, 5 by vitrification (V), and 5 by slow-freezing (SF). Pieces of fresh ovarian tissue were used for controls. After 1 month, the strips were autografted to two different vascular beds, healed (HB) or freshly decorticated (FDB), constituting four study groups: SF-HB, SF-FDB, V-HB, and V-FDB. These were compared to fresh tissue. After 6 months, the ovaries were removed and several parameters analyzed: follicle quality, stage, density, proliferation, apoptosis, functionality, va

SUBMITTER: Dolmans MM 

PROVIDER: S-EPMC4554387 | biostudies-literature | 2015 Aug

REPOSITORIES: biostudies-literature