Hyperglycemia promotes tissue plasminogen activator-induced hemorrhage by Increasing superoxide production.

ABSTRACT:

Objective

Risk of intracerebral hemorrhage is the primary factor limiting use of tissue plasminogen activator (tPA) for stroke. Clinical studies have established an association between admission hyperglycemia and the risk of hemorrhage with tPA use, independent of prior diabetes. Here we used an animal model of tPA-induced reperfusion hemorrhage to determine if this clinical association reflects a true causal relationship.

Methods

Rats underwent 90 minutes of focal ischemia, and tPA infusion was begun 10 minutes prior to vessel reperfusion. Glucose was administered during ischemia to generate blood levels ranging from 5.9 ± 1.8mM (normoglycemia) to 21 ± 2.3mM. In some studies, apocynin was administered to block superoxide production by nicotinamide adenine dinucleotide phosp

SUBMITTER: Won SJ 

PROVIDER: S-EPMC4554391 | biostudies-literature | 2011 Oct

REPOSITORIES: biostudies-literature