Scaffold-fused riboregulators for enhanced gene activation in Synechocystis sp. PCC 6803.

ABSTRACT: Cyanobacteria are an attractive host for biofuel production because they can produce valuable chemical compounds from CO2 fixed by photosynthesis. However, the available genetic tools that enable precise gene regulation for the applications of synthetic biology are insufficient. Previously, we engineered an RNA-based posttranscriptional regulator, termed riboregulator, for the control of target gene expression in cyanobacterium Synechocystis sp. PCC 6803. Moreover, we enhanced the gene regulation ability of the riboregulators in Escherichia coli by fusing and engineering a scaffold sequence derived from naturally occurring E. coli noncoding small RNAs. Here, we demonstrated that the scaffold sequence fused to the riboregulators improved their gene regulation ability in Synechocystis sp. PC

SUBMITTER: Sakai Y 

PROVIDER: S-EPMC4554450 | biostudies-literature | 2015 Aug

REPOSITORIES: biostudies-literature