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Analysis of the key enzymes of butyric and acetic acid fermentation in biogas reactors.

ABSTRACT: This study aimed at the investigation of the mechanisms of acidogenesis, which is a key process during anaerobic digestion. To expose possible bottlenecks, specific activities of the key enzymes of acidification, such as acetate kinase (Ack, 0.23-0.99?U mg(-1) protein), butyrate kinase (Buk,

SUBMITTER: Gabris C 

PROVIDER: S-EPMC4554474 | biostudies-literature | 2015 Sep

REPOSITORIES: biostudies-literature

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Analysis of the key enzymes of butyric and acetic acid fermentation in biogas reactors.

Gabris Christina C   Bengelsdorf Frank R FR   Dürre Peter P  

Microbial biotechnology 20150618 5

This study aimed at the investigation of the mechanisms of acidogenesis, which is a key process during anaerobic digestion. To expose possible bottlenecks, specific activities of the key enzymes of acidification, such as acetate kinase (Ack, 0.23-0.99 U mg(-1) protein), butyrate kinase (Buk, < 0.03 U mg(-1) protein) and butyryl-CoA:acetate-CoA transferase (But, 3.24-7.64 U mg(-1) protein), were determined in cell free extracts of biogas reactor content from three different biogas reactors. Furth  ...[more]

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