Transgalactosylating β-galactosidase from probiotic Lactobacillus plantarum MCC2156: production and permeabilization for use as whole cell biocatalyst.

ABSTRACT: Key nutritional factors were optimized for the maximum production of transgalactosylating β-galactosidase from Lactobacillus plantarum MCC2156. Galactose, yeast extract, sodium acetate and manganese sulphate were the most important nutrients affecting β-galactosidase production. Maximum β-galactosidase production (3015 miller units) was obtained by culturing L. plantarum in the optimized fermentation medium containing (w/v) galactose (4 %), yeast extract (2 %), sodium acetate (3 %) and manganese sulphate (0.075 %) with an optimum medium pH of 7.0, after 14 h of incubation at 35 °C. Further, permeabilization of L. plantarum cells using various chemical/ solvents for maximum β-galactosidase activity was performed for use as whole cell biocatalyst. Mixture of ethanol: n-butanol was found to e

SUBMITTER: Gobinath D 

PROVIDER: S-EPMC4554668 | biostudies-literature | 2015 Sep

REPOSITORIES: biostudies-literature