Comparative Genomics Reveals Chd1 as a Determinant of Nucleosome Spacing in Vivo.

ABSTRACT: Packaging of genomic DNA into nucleosomes is nearly universally conserved in eukaryotes, and many features of the nucleosome landscape are quite conserved. Nonetheless, quantitative aspects of nucleosome packaging differ between species because, for example, the average length of linker DNA between nucleosomes can differ significantly even between closely related species. We recently showed that the difference in nucleosome spacing between two Hemiascomycete species-Saccharomyces cerevisiae and Kluyveromyces lactis-is established by trans-acting factors rather than being encoded in cis in the DNA sequence. Here, we generated several S. cerevisiae strains in which endogenous copies of candidate nucleosome spacing factors are deleted and replaced with the orthologous factors from K. lactis.

SUBMITTER: Hughes AL 

PROVIDER: S-EPMC4555225 | biostudies-literature | 2015 Jul

REPOSITORIES: biostudies-literature