Inhibition of Rho-associated kinases disturbs the collective cell migration of stratified TE-10 cells.

ABSTRACT:

Background

The collective cell migration of stratified epithelial cells is considered to be an important phenomenon in wound healing, development, and cancer invasion; however, little is known about the mechanisms involved. Furthermore, whereas Rho family proteins, including RhoA, play important roles in cell migration, the exact role of Rho-associated coiled coil-containing protein kinases (ROCKs) in cell migration is controversial and might be cell-type dependent. Here, we report the development of a novel modified scratch assay that was used to observe the collective cell migration of stratified TE-10 cells derived from a human esophageal cancer specimen.

Results

Desmosomes were found between the TE-10 cells and microvilli of the surface of the cell sheet. The leading edg

SUBMITTER: Mikami T 

PROVIDER: S-EPMC4556056 | biostudies-literature | 2015 Sep

REPOSITORIES: biostudies-literature