High-content assays for characterizing the viability and morphology of 3D cancer spheroid cultures.

ABSTRACT: There is an increasing interest in using three-dimensional (3D) spheroids for modeling cancer and tissue biology to accelerate translation research. Development of higher throughput assays to quantify phenotypic changes in spheroids is an active area of investigation. The goal of this study was to develop higher throughput high-content imaging and analysis methods to characterize phenotypic changes in human cancer spheroids in response to compound treatment. We optimized spheroid cell culture protocols using low adhesion U-bottom 96- and 384-well plates for three common cancer cell lines and improved the workflow with a one-step staining procedure that reduces assay time and minimizes variability. We streamlined imaging acquisition by using a maximum projection algorithm that combines cell

SUBMITTER: Sirenko O 

PROVIDER: S-EPMC4556086 | biostudies-literature | 2015 Sep

REPOSITORIES: biostudies-literature