Integrin Based Isolation Enables Purification of Murine Lineage Committed Cardiomyocytes.

ABSTRACT: In contrast to mature cardiomyocytes which have limited regenerative capacity, pluripotent stem cells represent a promising source for the generation of new cardiomyocytes. The tendency of pluripotent stem cells to form teratomas and the heterogeneity from various differentiation stages and cardiomyocyte cell sub-types, however, are major obstacles to overcome before this type of therapy could be applied in a clinical setting. Thus, the identification of extracellular markers for specific cardiomyocyte progenitors and mature subpopulations is of particular importance. The delineation of cardiomyocyte surface marker patterns not only serves as a means to derive homogeneous cell populations by FACS, but is also an essential tool to understand cardiac development. By using single-cell express

SUBMITTER: Tarnawski L 

PROVIDER: S-EPMC4556377 | biostudies-literature | 2015

REPOSITORIES: biostudies-literature