Hierarchical maturation of innate immune defences in very preterm neonates.

ABSTRACT:

Background

Preterm neonates are highly vulnerable to infection.

Objectives

To investigate the developmental contribution of prematurity, chorioamnionitis and antenatal corticosteroids (ANS) on the maturation of neonatal microbial pathogen recognition responses.

Methods

Using standardized protocols, we assayed multiple inflammatory cytokine responses (IL-1β, IL-6, TNF-α and IL-12/23p40) to three prototypic Toll-like receptor (TLR) agonists, i.e. TLR4 (lipopolysaccharide), TLR5 (flagellin) and TLR7/8 (R848), and to the non-TLR retinoic acid-inducible gene I (RIG-I)-like receptor agonist, in cord blood mononuclear cells from neonates born before 33 weeks of gestation and at term.

Results

TLR responses develop asynchronously in preterm neonates, whereby responses t

SUBMITTER: Sharma AA 

PROVIDER: S-EPMC4556450 | biostudies-literature | 2014

REPOSITORIES: biostudies-literature