Cullin4 Is Pro-Viral during West Nile Virus Infection of Culex Mosquitoes.

ABSTRACT: Although mosquitoes serve as vectors of many pathogens of public health importance, their response to viral infection is poorly understood. It also remains to be investigated whether viruses deploy some mechanism to be able to overcome this immune response. Here, we have used an RNA-Seq approach to identify differentially regulated genes in Culex quinquefasciatus cells following West Nile virus (WNV) infection, identifying 265 transcripts from various cellular pathways that were either upregulated or downregulated. Ubiquitin-proteasomal pathway genes, comprising 12% of total differentially regulated genes, were selected for further validation by real time RT-qPCR and functional analysis. It was found that treatment of infected cells with proteasomal inhibitor, MG-132, decreased WNV titers,

SUBMITTER: Paradkar PN 

PROVIDER: S-EPMC4556628 | biostudies-literature | 2015 Sep

REPOSITORIES: biostudies-literature