Tracking Dengue Virus Intra-host Genetic Diversity during Human-to-Mosquito Transmission.

ABSTRACT: Dengue virus (DENV) infection of an individual human or mosquito host produces a dynamic population of closely-related sequences. This intra-host genetic diversity is thought to offer an advantage for arboviruses to adapt as they cycle between two very different host species, but it remains poorly characterized. To track changes in viral intra-host genetic diversity during horizontal transmission, we infected Aedes aegypti mosquitoes by allowing them to feed on DENV2-infected patients. We then performed whole-genome deep-sequencing of human- and matched mosquito-derived DENV samples on the Illumina platform and used a sensitive variant-caller to detect single nucleotide variants (SNVs) within each sample. >90% of SNVs were lost upon transition from human to mosquito, as well as from mosqui

SUBMITTER: Sim S 

PROVIDER: S-EPMC4556672 | biostudies-literature | 2015

REPOSITORIES: biostudies-literature