Fluorescence In Situ Hybridization (FISH) Assays for Diagnosing Malaria in Endemic Areas.

ABSTRACT: Malaria is a responsible for approximately 600 thousand deaths worldwide every year. Appropriate and timely treatment of malaria can prevent deaths but is dependent on accurate and rapid diagnosis of the infection. Currently, microscopic examination of the Giemsa stained blood smears is the method of choice for diagnosing malaria. Although it has limited sensitivity and specificity in field conditions, it still remains the gold standard for the diagnosis of malaria. Here, we report the development of a fluorescence in situ hybridization (FISH) based method for detecting malaria infection in blood smears and describe the use of an LED light source that makes the method suitable for use in resource-limited malaria endemic countries. The Plasmodium Genus (P-Genus) FISH assay has a Plasmodium

SUBMITTER: Shah J 

PROVIDER: S-EPMC4558036 | biostudies-literature | 2015

REPOSITORIES: biostudies-literature