Hematopoietic progenitors are required for proper development of coronary vasculature.

ABSTRACT:

Rationale

During embryogenesis, hematopoietic cells appear in the myocardium prior to the initiation of coronary formation. However, their role is unknown.

Objective

Here we investigate whether pre-existing hematopoietic cells are required for the formation of coronary vasculature.

Methods and results

As a model of for hematopoietic cell deficient animals, we used Runx1 knockout embryos and Vav1-cre; R26-DTA embryos, latter of which genetically ablates 2/3 of CD45(+) hematopoietic cells. Both Runx1 knockout embryos and Vav1-cre; R26-DTA embryos revealed disorganized, hypoplastic microvasculature of coronary vessels on section and whole-mount stainings. Furthermore, coronary explant experiments showed that the mouse heart explants from Runx1 and Vav1-cre; R26-DTA embry

SUBMITTER: Lluri G 

PROVIDER: S-EPMC4558312 | biostudies-literature | 2015 Sep

REPOSITORIES: biostudies-literature