Immortalization and characterization of mouse temporomandibular joint disc cell clones with capacity for multi-lineage differentiation.

ABSTRACT:

Objective

Despite the importance of temporomandibular joint (TMJ) disc in normal function and disease, studying the responses of its cells has been complicated by the lack of adequate characterization of the cell subtypes. The purpose of our investigation was to immortalize, clone, characterize and determine the multi-lineage potential of mouse TMJ disc cells.

Design

Cells from 12-week-old female mice were cultured and immortalized by stable transfection with human telomerase reverse transcriptase (hTERT). The immortalized cell clones were phenotyped for fibroblast- or chondrocyte-like characteristics and ability to undergo adipocytic, osteoblastic and chondrocytic differentiation.

Results

Of 36 isolated clones, four demonstrated successful immortalization and mainten

SUBMITTER: Park Y 

PROVIDER: S-EPMC4558381 | biostudies-literature | 2015 Sep

REPOSITORIES: biostudies-literature