Progenitor cell expansion and impaired hepatocyte regeneration in explanted livers from alcoholic hepatitis.

ABSTRACT:

Objective

In alcoholic hepatitis (AH), development of targeted therapies is crucial and requires improved knowledge of cellular and molecular drivers in liver dysfunction. The unique opportunity of using explanted livers from patients with AH having undergone salvage liver transplantation allowed to perform more in-depth molecular translational studies.

Design

We studied liver explants from patients with AH submitted to salvage transplantation (n=16), from patients with alcoholic cirrhosis without AH (n=12) and fragments of normal livers (n=16). Hepatic cytokine content was quantified. Hepatocyte function and proliferation and the presence of hepatic progenitor cells (HPCs) were evaluated by immunohistochemistry, western blot or quantitative PCR. Mitochondrial morphology was

SUBMITTER: Dubuquoy L 

PROVIDER: S-EPMC4558407 | biostudies-literature | 2015 Dec

REPOSITORIES: biostudies-literature