High-resolution non-contact measurement of the electrical activity of plants in situ using optical recording.

ABSTRACT: The limitations of conventional extracellular recording and intracellular recording make high-resolution multisite recording of plant bioelectrical activity in situ challenging. By combining a cooled charge-coupled device camera with a voltage-sensitive dye, we recorded the action potentials in the stem of Helianthus annuus and variation potentials at multiple sites simultaneously with high spatial resolution. The method of signal processing using coherence analysis was used to determine the synchronization of the selected signals. Our results provide direct visualization of the phloem, which is the distribution region of the electrical activities in the stem and leaf of H. annuus, and verify that the phloem is the main action potential transmission route in the stems of higher plants. Fin

SUBMITTER: Zhao DJ 

PROVIDER: S-EPMC4558603 | biostudies-literature | 2015 Sep

REPOSITORIES: biostudies-literature