Identification of genes associated with methotrexate resistance in methotrexate-resistant osteosarcoma cell lines.

ABSTRACT:

Background

This study aimed to better understand the mechanisms underlying methotrexate (MTX)-resistance in osteosarcoma.

Methods

The raw transcription microarray data GSE16089 collected from three MTX-sensitive osteosarcoma (Saos-2) cell samples and three MTX-resistant osteosarcoma (Saos-2) cell samples were downloaded from Gene Expression Omnibus. After data processing, the differentially expressed genes (DEGs) were identified. Next, DEGs were submitted to DAVID for functional annotation based on the GO (Gene Ontology) database, as well as pathway enrichment analysis based on the KEGG (Kyoto Encyclopedia of Genes and Genomes) database. Transcription factors (TFs) and tumor-associated genes (TAGs) were identified with reference to TRANSFAC and TAG, and TSGene databases, res

SUBMITTER: Yang XR 

PROVIDER: S-EPMC4558632 | biostudies-literature | 2015 Sep

REPOSITORIES: biostudies-literature