Evolutionary Divergence of Gene and Protein Expression in the Brains of Humans and Chimpanzees.

ABSTRACT: Although transcriptomic profiling has become the standard approach for exploring molecular differences in the primate brain, very little is known about how the expression levels of gene transcripts relate to downstream protein abundance. Moreover, it is unknown whether the relationship changes depending on the brain region or species under investigation. We performed high-throughput transcriptomic (RNA-Seq) and proteomic (liquid chromatography coupled with tandem mass spectrometry) analyses on two regions of the human and chimpanzee brain: The anterior cingulate cortex and caudate nucleus. In both brain regions, we found a lower correlation between mRNA and protein expression levels in humans and chimpanzees than has been reported for other tissues and cell types, suggesting that the brain

SUBMITTER: Bauernfeind AL 

PROVIDER: S-EPMC4558850 | biostudies-literature | 2015 Jul

REPOSITORIES: biostudies-literature