Widespread Recurrent Patterns of Rapid Repeat Evolution in the Kinetochore Scaffold KNL1.

ABSTRACT: The outer kinetochore protein scaffold KNL1 is essential for error-free chromosome segregation during mitosis and meiosis. A critical feature of KNL1 is an array of repeats containing MELT-like motifs. When phosphorylated, these motifs form docking sites for the BUB1-BUB3 dimer that regulates chromosome biorientation and the spindle assembly checkpoint. KNL1 homologs are strikingly different in both the amount and sequence of repeats they harbor. We used sensitive repeat discovery and evolutionary reconstruction to show that the KNL1 repeat arrays have undergone extensive, often species-specific array reorganization through iterative cycles of higher order multiplication in conjunction with rapid sequence diversification. The number of repeats per array ranges from none in flowering plants

SUBMITTER: Tromer E 

PROVIDER: S-EPMC4558858 | biostudies-literature | 2015 Aug

REPOSITORIES: biostudies-literature