Evaluation of six novel antigens as potential biomarkers for the early immunodiagnosis of schistosomiasis.

ABSTRACT:

Background

Early diagnosis of schistosomiasis, prior to egg laying, would enable earlier treatment and help interrupt the transmission cycle of the parasite and the progress of the disease. Previously we identified six novel antigens with potential as diagnostic markers for human Schistosoma japonicum infections. In this study, we evaluated these antigens as candidate biomarkers for the early diagnosis of schistosomiasis in mice and rabbits.

Methods

The transcriptional profiles of the six antigens (SjSP-13, SjSP-23, SjSP-160, SjSP-164, SjSP-189 and SjSP-216) at different developmental stages were analyzed by quantitative PCR. The recombinant proteins were expressed in E. coli and purified with nickel chelate affinity chromatography. We then developed recombinant protein-base

SUBMITTER: Zhang Y 

PROVIDER: S-EPMC4558877 | biostudies-literature | 2015 Sep

REPOSITORIES: biostudies-literature