Development of monoclonal antibodies and serological assays specific for Barley yellow dwarf virus GAV strain.

ABSTRACT:

Background

Barley yellow dwarf virus (BYDV) is one of the most devastating plant viruses and belongs to a ubiquitous plant virus group. In China, four BYDV strains (GPV, GAV, PAV and RMV) have been identified based on their specific aphid vectors and serological properties. Among the four identified strains, the GAV is the most common BYDV strain in China. To diagnose, forecast of BYDV GAV, two reliable serological assays for BYDV GAV detection were established.

Methods

We purified virion from a confirmed BYDV GAV source and used it as the immunogen to produce monoclonal antibodies against the virus. Using the hybridoma technology, three highly specific murine monoclonal antibodies were produced and two serological assays [antigen-coated-plate enzyme-linked immunosorbent ass

SUBMITTER: Li N 

PROVIDER: S-EPMC4558997 | biostudies-literature | 2015 Sep

REPOSITORIES: biostudies-literature