Comparison of stranded and non-stranded RNA-seq transcriptome profiling and investigation of gene overlap.

ABSTRACT:

Background

While RNA-sequencing (RNA-seq) is becoming a powerful technology in transcriptome profiling, one significant shortcoming of the first-generation RNA-seq protocol is that it does not retain the strand specificity of origin for each transcript. Without strand information it is difficult and sometimes impossible to accurately quantify gene expression levels for genes with overlapping genomic loci that are transcribed from opposite strands. It has recently become possible to retain the strand information by modifying the RNA-seq protocol, known as strand-specific or stranded RNA-seq. Here, we evaluated the advantages of stranded RNA-seq in transcriptome profiling of whole blood RNA samples compared with non-stranded RNA-seq, and investigated the influence of gene overlaps on

SUBMITTER: Zhao S 

PROVIDER: S-EPMC4559181 | biostudies-literature | 2015 Sep

REPOSITORIES: biostudies-literature