Zoster Vaccination Increases the Breadth of CD4+ T Cells Responsive to Varicella Zoster Virus.
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ABSTRACT: Background
The live, attenuated varicella vaccine strain (vOka) is the only licensed therapeutic vaccine. Boost of varicella zoster virus (VZV)-specific cellular immunity is a likely mechanism of action. We examined memory CD4(+) T-cell responses to each VZV protein at baseline and after zoster vaccination.Methods
Serial blood samples were collected from 12 subjects vaccinated with Zostavax and immunogenicity confirmed by ex vivo VZV-specific T-cell and antibody assays. CD4(+) T-cell lines enriched for VZV specificity were generated and probed for proliferative responses to every VZV protein and selected peptide sets.Results
Zoster vaccination increased the median magnitude (2.3-fold) and breadth (4.2-fold) of VZV-specific CD4(+) T cells one month post-vaccination. Both measures declined by 6 months. The most prevalent responses at baseline included VZV open reading frames (ORFs) 68, 4, 37, and 63. After vaccination, responses to ORFs 40, 67, 9, 59, 12, 62, and 18 were also prevalent. The immunogenicity of ORF9 and ORF18 were confirmed using peptides, defining a large number of discrete CD4 T-cell epitopes.Conclusions
The breadth and magnitude of the VZV-specific CD4(+) T-cell response increase after zoster vaccination. In addition to glycoprotein E (ORF68), we identified antigenic ORFs that may be useful components of subunit vaccines.
SUBMITTER: Laing KJ
PROVIDER: S-EPMC4559191 | biostudies-literature | 2015 Oct
REPOSITORIES: biostudies-literature
Publications
Zoster Vaccination Increases the Breadth of CD4+ T Cells Responsive to Varicella Zoster Virus.
Laing Kerry J KJ Russell Ronnie M RM Dong Lichun L Schmid D Scott DS Stern Michael M Magaret Amalia A Haas Jürgen G JG Johnston Christine C Wald Anna A Koelle David M DM
The Journal of infectious diseases 20150317 7
<h4>Background</h4>The live, attenuated varicella vaccine strain (vOka) is the only licensed therapeutic vaccine. Boost of varicella zoster virus (VZV)-specific cellular immunity is a likely mechanism of action. We examined memory CD4(+) T-cell responses to each VZV protein at baseline and after zoster vaccination.<h4>Methods</h4>Serial blood samples were collected from 12 subjects vaccinated with Zostavax and immunogenicity confirmed by ex vivo VZV-specific T-cell and antibody assays. CD4(+) T- ...[more]
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