Characterizing 5-hydroxymethylcytosine in human prefrontal cortex at single base resolution.

ABSTRACT:

Background

The recent discovery that methylated cytosines are converted to 5-hydroxymethylated cytosines (5hmC) by the family of ten-eleven translocation enzymes has sparked significant interest on the genomic location, the abundance in different tissues, the putative functions, and the stability of this epigenetic mark. 5hmC plays a key role in the brain, where it is particularly abundant and dynamic during development.

Results

Here, we comprehensively characterize 5hmC in the prefrontal cortices of 24 subjects. We show that, although there is inter-individual variability in 5hmC content among unrelated individuals, approximately 8 % of all CpGs on autosomal chromosomes contain 5hmC, while sex chromosomes contain far less. Our data also provide evidence suggesting that 5hmC

SUBMITTER: Gross JA 

PROVIDER: S-EPMC4559220 | biostudies-literature | 2015 Sep

REPOSITORIES: biostudies-literature