Patient-derived heavy chain antibody targets cell surface HSP90 on breast tumors.

ABSTRACT:

Background

Monoclonal antibodies have been used to effectively treat various tumors. We previously established a unique strategy to identify tumor specific antibodies by capturing B-cell response against breast tumor antigens from patient-derived sentinel lymph nodes. Initial application of this approach led to identification of a tumor specific single domain antibody. In this paper we optimized our previous strategy by generating heavy chain antibodies (HCAbs) to overcome the deficiencies of single domain antibodies. Here we identified and characterized a heavy chain antibody (HCAb2) that targets cell surface HSP90 antigen on breast tumor cells but not normal cells.

Methods

Eight HCAbs derived from 4 breast cancer patients were generated using an in vitro expression system.

SUBMITTER: Devarakonda CV 

PROVIDER: S-EPMC4559304 | biostudies-literature | 2015 Sep

REPOSITORIES: biostudies-literature