Comparison of a Real-Time Multiplex PCR and Sequetyping Assay for Pneumococcal Serotyping.

ABSTRACT:

Background

Pneumococcal serotype identification is essential to monitor pneumococcal vaccine effectiveness and serotype replacement. Serotyping by conventional serological methods are costly, labour-intensive, and require significant technical expertise. We compared two different molecular methods to serotype pneumococci isolated from the nasopharynx of South African infants participating in a birth cohort study, the Drakenstein Child Health Study, in an area with high 13-valent pneumococcal conjugate vaccine (PCV13) coverage.

Methods

A real-time multiplex PCR (rmPCR) assay detecting 21 different serotypes/-groups and a sequetyping assay, based on the sequence of the wzh gene within the pneumococcal capsular locus, were compared. Forty pneumococcal control isolates, with ser

SUBMITTER: Dube FS 

PROVIDER: S-EPMC4559314 | biostudies-literature | 2015

REPOSITORIES: biostudies-literature