Role of miRNAs in neuronal differentiation from human embryonic stem cell-derived neural stem cells.

ABSTRACT: microRNAs (miRNAs) are important modulators in regulating gene expression at the post-transcriptional level and are therefore emerging as strong mediators in neural fate determination. Here, by use of the model of human embryonic stem cell (hESC)-derived neurogenesis, miRNAs involved in the differentiation from neural stem cells (hNSC) to neurons were profiled and identified. hNSC were differentiated into the neural lineage, out of which the neuronal subset was enriched through cell sorting based on select combinatorial biomarkers: CD15-/CD29(Low)/CD24(High). This relatively pure and viable subpopulation expressed the neuronal marker β III-tubulin. The miRNA array demonstrated that a number of miRNAs were simultaneously induced or suppressed in neurons, as compared to hNSC. Real-time PCR f

SUBMITTER: Liu J 

PROVIDER: S-EPMC4559349 | biostudies-literature | 2012 Dec

REPOSITORIES: biostudies-literature