High glucose inhibits ClC-2 chloride channels and attenuates cell migration of rat keratinocytes.

ABSTRACT:

Background

Accumulating evidence has demonstrated that migration of keratinocytes is critical to wound epithelialization, and defects of this function result in chronic delayed-healing wounds in diabetes mellitus patients, and the migration has been proved to be associated with volume-activated chloride channels. The aim of the study is to investigate the effects of high glucose (HG, 25 mM) on ClC-2 chloride channels and cell migration of keratinocytes.

Methods

Newborn Sprague Dawley rats were used to isolate and culture the keratinocyte in this study. Immunofluorescence assay, real-time polymerase chain reaction, and Western blot assay were used to examine the expression of ClC-2 protein or mRNA. Scratch wound assay was used to measure the migratory ability of keratinocytes

SUBMITTER: Pan F 

PROVIDER: S-EPMC4560522 | biostudies-literature | 2015

REPOSITORIES: biostudies-literature