Expression and subcellular localization of the Qa-SNARE syntaxin17 in human eosinophils.

ABSTRACT:

Background

SNARE members mediate membrane fusion during intracellular trafficking underlying innate and adaptive immune responses by different cells. However, little is known about the expression and function of these proteins in human eosinophils, cells involved in allergic, inflammatory and immunoregulatory responses. Here, we investigate the expression and distribution of the Qa-SNARE syntaxin17 (STX17) within human eosinophils isolated from the peripheral blood.

Methods

Flow cytometry and a pre-embedding immunonanogold electron microscopy (EM) technique that combines optimal epitope preservation and secondary Fab-fragments of antibodies linked to 1.4 nm gold particles for optimal access to microdomains, were used to investigate STX17.

Results

STX17 was detected wi

SUBMITTER: Carmo LAS 

PROVIDER: S-EPMC4561602 | biostudies-literature | 2015 Oct

REPOSITORIES: biostudies-literature