High content analysis at single cell level identifies different cellular responses dependent on nanomaterial concentrations.

ABSTRACT: A mechanistic understanding of nanomaterial (NM) interaction with biological environments is pivotal for the safe transition from basic science to applied nanomedicine. NM exposure results in varying levels of internalized NM in different neighboring cells, due to variances in cell size, cell cycle phase and NM agglomeration. Using high-content analysis, we investigated the cytotoxic effects of fluorescent quantum dots on cultured cells, where all effects were correlated with the concentration of NMs at the single cell level. Upon binning the single cell data into different categories related to NM concentration, this study demonstrates, for the first time, that quantum dots activate both cytoprotective and cytotoxic mechanisms, resulting in a zero net result on the overall cell population

SUBMITTER: Manshian BB 

PROVIDER: S-EPMC4561960 | biostudies-literature | 2015 Sep

REPOSITORIES: biostudies-literature