Tentacle: distributed quantification of genes in metagenomes.

ABSTRACT:

Background

In metagenomics, microbial communities are sequenced at increasingly high resolution, generating datasets with billions of DNA fragments. Novel methods that can efficiently process the growing volumes of sequence data are necessary for the accurate analysis and interpretation of existing and upcoming metagenomes.

Findings

Here we present Tentacle, which is a novel framework that uses distributed computational resources for gene quantification in metagenomes. Tentacle is implemented using a dynamic master-worker approach in which DNA fragments are streamed via a network and processed in parallel on worker nodes. Tentacle is modular, extensible, and comes with support for six commonly used sequence aligners. It is easy to adapt Tentacle to different applications in

SUBMITTER: Boulund F 

PROVIDER: S-EPMC4562114 | biostudies-literature | 2015

REPOSITORIES: biostudies-literature