Comparative analyses of CTCF and BORIS occupancies uncover two distinct classes of CTCF binding genomic regions.

ABSTRACT:

Background

CTCF and BORIS (CTCFL), two paralogous mammalian proteins sharing nearly identical DNA binding domains, are thought to function in a mutually exclusive manner in DNA binding and transcriptional regulation.

Results

Here we show that these two proteins co-occupy a specific subset of regulatory elements consisting of clustered CTCF binding motifs (termed 2xCTSes). BORIS occupancy at 2xCTSes is largely invariant in BORIS-positive cancer cells, with the genomic pattern recapitulating the germline-specific BORIS binding to chromatin. In contrast to the single-motif CTCF target sites (1xCTSes), the 2xCTS elements are preferentially found at active promoters and enhancers, both in cancer and germ cells. 2xCTSes are also enriched in genomic regions that escape histone to p

SUBMITTER: Pugacheva EM 

PROVIDER: S-EPMC4562119 | biostudies-literature | 2015 Aug

REPOSITORIES: biostudies-literature