Draft genome of Brugia pahangi: high similarity between B. pahangi and B. malayi.

ABSTRACT:

Background

Efforts to completely eradicate lymphatic filariasis from human population may be challenged by the emergence of Brugia pahangi as another zoonotic lymphatic filarial nematode. In this report, a genomic study was conducted to understand this species at molecular level.

Methods

After blood meal on a B. pahangi-harbouring cat, the Aedes togoi mosquitoes were maintained to harvest infective third stage larvae, which were then injected into male Mongolian gerbils. Subsequently, adult B. pahangi were obtained from the infected gerbil for genomic DNA extraction. Sequencing and subsequently, construction of genomic libraries were performed. This was followed by genomic analyses and gene annotation analysis. By using archived protein sequences of B. malayi and a few other

SUBMITTER: Lau YL 

PROVIDER: S-EPMC4562187 | biostudies-literature | 2015 Sep

REPOSITORIES: biostudies-literature