High-resolution profiling of Drosophila replication start sites reveals a DNA shape and chromatin signature of metazoan origins.

ABSTRACT: At every cell cycle, faithful inheritance of metazoan genomes requires the concerted activation of thousands of DNA replication origins. However, the genetic and chromatin features defining metazoan replication start sites remain largely unknown. Here, we delineate the origin repertoire of the Drosophila genome at high resolution. We address the role of origin-proximal G-quadruplexes and suggest that they transiently stall replication forks in vivo. We dissect the chromatin configuration of replication origins and identify a rich spatial organization of chromatin features at initiation sites. DNA shape and chromatin configurations, not strict sequence motifs, mark and predict origins in higher eukaryotes. We further examine the link between transcription and origin firing and reveal that m

SUBMITTER: Comoglio F 

PROVIDER: S-EPMC4562395 | biostudies-literature | 2015 May

REPOSITORIES: biostudies-literature