Feasibility of protein turnover studies in prototroph Saccharomyces cerevisiae strains.

ABSTRACT: Quantitative proteomics studies of yeast that use metabolic labeling with amino acids rely on auxotrophic mutations of one or more genes on the amino acid biosynthesis pathways. These mutations affect yeast metabolism and preclude the study of some biological processes. Overcoming this limitation, it has recently been described that proteins in a yeast prototrophic strain can also be metabolically labeled with heavy amino acids. However, the temporal profiles of label incorporation under the different phases of the prototroph's growth have not been examined. Labeling trajectories are important in the study of protein turnover and dynamics, in which label incorporation into proteins is monitored across many time points. Here we monitored protein labeling trajectories for 48 h after a pulse

SUBMITTER: Martin-Perez M 

PROVIDER: S-EPMC4562466 | biostudies-literature | 2015 Apr

REPOSITORIES: biostudies-literature