The novel cardiac myosin activator omecamtiv mecarbil increases the calcium sensitivity of force production in isolated cardiomyocytes and skeletal muscle fibres of the rat.

ABSTRACT:

Background and purpose

Omecamtiv mecarbil (OM) is a novel cardiac myosin activator drug for inotropic support in systolic heart failure. Here we have assessed the concentration-dependent mechanical effects of OM in permeabilized cardiomyocyte-sized preparations and single skeletal muscle fibres of Wistar-Kyoto rats under isometric conditions.

Experimental approaches

Ca²⁺ -dependent active force production (F_active ), its Ca²⁺ sensitivity (pCa₅₀ ), the kinetic characteristics of Ca²⁺ -regulated activation and relaxation, and Ca²⁺ -independent passive force (F_passive ) were monitored in Triton X-100-skinned preparations with and without OM (3nM-10 μM).

Key results

In permeabilized cardiomyocyte

SUBMITTER: Nagy L 

PROVIDER: S-EPMC4562511 | biostudies-literature | 2015 Sep

REPOSITORIES: biostudies-literature