Dysregulated post-transcriptional control of COX-2 gene expression in gestational diabetic endothelial cells.

ABSTRACT:

Background and purpose

Hyperglycaemic memory describes the progression of diabetic complications during subsequent periods of improved glycaemia. We addressed the hypothesis that transient hyperglycaemia causes aberrant COX-2 expression in HUVEC in response to IL-1β through the induction of long-lasting epigenetic changes involving microRNA-16 (miR-16), a post-transcriptional modulator of COX-2 expression.

Experimental approach

Studies were performed on HUVEC collected from women with gestational diabetes mellitus (GDM) (dHUVEC) and normal women (nHUVEC).

Key results

In dHUVEC treated with IL-1β, the expression of COX-2 mRNA and protein was enhanced and generation of prostanoids increased (the most abundant was the promitogenic PGF_2α ). COX-2 mRNA was more

SUBMITTER: Di Francesco L 

PROVIDER: S-EPMC4562516 | biostudies-literature | 2015 Sep

REPOSITORIES: biostudies-literature