Proportional-Integral-Derivative (PID) Control of Secreted Factors for Blood Stem Cell Culture.

ABSTRACT: Clinical use of umbilical cord blood has typically been limited by the need to expand hematopoietic stem and progenitor cells (HSPC) ex vivo. This expansion is challenging due to the accumulation of secreted signaling factors in the culture that have a negative regulatory effect on HSPC output. Strategies for global regulation of these factors through dilution have been developed, but do not accommodate the dynamic nature or inherent variability of hematopoietic cell culture. We have developed a mathematical model to simulate the impact of feedback control on in vitro hematopoiesis, and used it to design a proportional-integral-derivative (PID) control algorithm. This algorithm was implemented with a fed-batch bioreactor to regulate the concentrations of secreted factors. Controlling the c

SUBMITTER: Caldwell J 

PROVIDER: S-EPMC4562642 | biostudies-literature | 2015

REPOSITORIES: biostudies-literature