The AMPK activator R419 improves exercise capacity and skeletal muscle insulin sensitivity in obese mice.

ABSTRACT:

Objective

Skeletal muscle AMP-activated protein kinase (AMPK) is important for regulating glucose homeostasis, mitochondrial content and exercise capacity. R419 is a mitochondrial complex-I inhibitor that has recently been shown to acutely activate AMPK in myotubes. Our main objective was to examine whether R419 treatment improves insulin sensitivity and exercise capacity in obese insulin resistant mice and whether skeletal muscle AMPK was important for mediating potential effects.

Methods

Glucose homeostasis, insulin sensitivity, exercise capacity, and electron transport chain content/activity were examined in wildtype (WT) and AMPK β1β2 muscle-specific null (AMPK-MKO) mice fed a high-fat diet (HFD) with or without R419 supplementation.

Results

There was no change in

SUBMITTER: Marcinko K 

PROVIDER: S-EPMC4563030 | biostudies-literature | 2015 Sep

REPOSITORIES: biostudies-literature