Comparison of digital PCR platforms and semi-nested qPCR as a tool to determine the size of the HIV reservoir.

ABSTRACT: HIV persists in latently infected cells of patients on antiretroviral therapy (ART). This persistent proviral DNA reservoir is an important predictor of viral rebound upon therapy failure or interruption and forms a major obstacle towards cure. Accurate quantification of the low levels of persisting HIV DNA may aid patient monitoring and cure research. Digital PCR is a promising tool that enables direct absolute quantification with high sensitivity. With recent technological advances, several platforms are available to implement digital PCR in a clinical setting. Here, we compared two digital PCR platforms, the Quantstudio 3D (Life Technologies) and the QX100 (Bio-Rad) with a semi-nested qPCR on serial HIV DNA dilutions and DNA isolated from PBMCs of ART-suppressed patients. All three meth

SUBMITTER: Bosman KJ 

PROVIDER: S-EPMC4563360 | biostudies-literature | 2015 Sep

REPOSITORIES: biostudies-literature