Less is More: Membrane Protein Digestion Beyond Urea-Trypsin Solution for Next-level Proteomics.

ABSTRACT: The goal of next-level bottom-up membrane proteomics is protein function investigation, via high-coverage high-throughput peptide-centric quantitation of expression, modifications and dynamic structures at systems scale. Yet efficient digestion of mammalian membrane proteins presents a daunting barrier, and prevalent day-long urea-trypsin in-solution digestion proved insufficient to reach this goal. Many efforts contributed incremental advances over past years, but involved protein denaturation that disconnected measurement from functional states. Beyond denaturation, the recent discovery of structure/proteomics omni-compatible detergent n-dodecyl-β-d-maltopyranoside, combined with pepsin and PNGase F columns, enabled breakthroughs in membrane protein digestion: a 2010 DDM-low-TCEP (DLT) m

SUBMITTER: Zhang X 

PROVIDER: S-EPMC4563727 | biostudies-literature | 2015 Sep

REPOSITORIES: biostudies-literature