The anti-inflammatory activities of ethanol extract from Dan-Lou prescription in vivo and in vitro.

ABSTRACT:

Background

Although, Dan-Lou prescription (DLP) is used for antagonizing check discomfort and heartache, the pharmacological mechanism has not been clearly illustrated. Our present study aimed to design inflammatory models induced by LPS in vivo and in vitro to investigate the anti-inflammation of DLP ethanol extract (EEDL) and the potential mechanisms.

Methods

EEDL was prepared and then analyzed by high performance liquid chromatography (HPLC). Further, the anti-inflammatory effects of EEDL in vivo was evaluated by measuring inflammation-associated factors includingcytokines, chemokines and acute phase proteins in lipopolysaccharide (LPS)-induced mice serum and liver. The anti-inflammatory mechanism exploration of EEDL was performed in LPS-stimulated RAW 264.7 cells. Differ

SUBMITTER: Gao LN 

PROVIDER: S-EPMC4563854 | biostudies-literature | 2015 Sep

REPOSITORIES: biostudies-literature